Mode of Inhibition of Diphtheria Toxin by Ammonium Chloride

Mode of inhibition of diphtheria toxin by ammonium chloride.

Kim, K. (University of Washington, Seattle), and N. B. Groman. Mode of inhibition of diphtheria toxin by ammonium chloride. J. Bacteriol. 90:1557-1562. 1965.-The inhibition of diphtheria toxin by ammonium salts was independent of toxin concentration over a 100-fold range of toxin. Inhibition by minimal concentrations of ammonium chloride was abolished by lowering the pH, indicating that free am...

In vitro inhibition of diphtheria toxin action by ammonium salts and amines.

Kim, K. (University of Washington, Seattle), and N. B. Groman. In vitro inhibition of diphtheria toxin action by ammonium salts and amines. J. Bacteriol. 90:1552-1556. 1965.-An inhibitor for diphtheria toxin action on HeLa cells was demonstrated in the growth supernatant fractions of both toxinogenic and nontoxinogenic strains of Corynebacterium diphtheriae and in the Mueller and Miller medium ...

Mode of Action of Diphtheria Toxin

In a preceding paper it was shown that low concentrations of diphtheria toxin, in the presence of NAD 1 as an essential cofactor, inhibit the incorporation of amino acids into TCA-precipitable peptides in mammalian cell extracts (1). Collier (2) has demonstrated that this inhibitory action of the toxin is due to the specific inactivation of transferase II , one of the highly labile soluble enzy...

Diphtheria toxin: mode of action and structure.

INTRODUCTION.... 54 OUTLINE OF THEPROBLEM. 54 INHIBITION OFPROTEIN SYNTHESIS ..56 Studies in Cell Culture ..56 Studies in Cell-Free Systems . 8 ADP-RIBOSYLATION OF EF-2 .59 Identification of the Reaction . ADPR is Covalently Attached . .60 The Role of Toxin is Catalytic . 1 Substrate Specificity ..6 Reversibility and Thermodynamics .62 Factors Affecting the Rate of the Forward Reaction 62 STRUC...

Preparation of a low-chloride Medium, for the production of Diphtheria toxin